Bio-Rad Western C Protein Ladder: A Comprehensive Guide

The Bio-Rad Western C Protein Ladder is a reliable and versatile tool used to analyze protein sizes in western blotting applications. This ladder consists of a range of protein standards, each with a unique molecular weight, making it an essential component for researchers to accurately determine the size of their proteins.

One of the key features of the Bio-Rad Western C Protein Ladder is its recommended volume per well for 1.0 mm mini gels. For electrophoresis, the recommended volume is 5 μL, while for blotting, it's 2 μL. This ensures that the ladder remains visible even after transfer to the blotted membrane.

The ladder itself consists of a range of protein standards with molecular weights ranging from ~10 kDa to ~250 kDa. Each standard contains a 6X His-tag, allowing for detection using the InVision His-tag In-gel Stain or other methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stains.

The Bio-Rad Western C Protein Ladder can be used in conjunction with various staining protocols, including Coomassie staining. This allows researchers to visualize the ladder and determine the size of their protein samples with ease. Additionally, the ladder can be detected using antibodies against Strep-tag II sequence, making it an ideal tool for analyzing tagged-proteins.

Another advantage of the Bio-Rad Western C Protein Ladder is its accuracy and precision. The ladder's molecular weights are precisely controlled, allowing researchers to accurately determine the size of their protein samples. This makes it an essential component for researchers working with high-molecular-weight proteins.

In conclusion, the Bio-Rad Western C Protein Ladder is a reliable and versatile tool used to analyze protein sizes in western blotting applications. Its range of molecular weights, precise control over its composition, and ease of detection make it an essential component for any researcher working with proteins.

Bio rad western c protein ladder